|
KMID : 0380620130450060801
|
|
Korean Journal of Food Science and Technology
2013 Volume.45 No. 6 p.801 ~ p.804
|
|
|
Production of Glutathione by the Yeast Mutant Saccharomyces cerevisiae Sa59
|
|
Jang Hye-Yoon
Oh Chul-Hwan
Oh Nam-Soon
|
|
|
Abstract
|
|
|
|
The glutathione contents of the selected mutants were investigated and found to be 6.1-15.8 mg/g-DCW. The glutathione content positively correlated with the antioxidant activity of the mutant strains (R2=0.488). Furthermore, the glutathione content of the mutant S. cerevisiae Sa-59 was approximately 38% greater than that of the wild type strain and, therefore, this mutant strain was selected for glutathione production. The volumetric glutathione content in a shaking culture was increased by about 70% compared to the static culture. In addition, the specific glutathione content was increased by ~19%. The volumetric glutathione content and specific glutathione content were increased by approximately 16% and 66%, respectively, when 0.04% glutamate, 0.04% cysteine and 0.04% glycine were added. Furthermore, the highest antioxidant activity was 0.52 as absorbance unit at 700 nm.
|
|
|
KEYWORD
|
|
|
glutathione, yeast mutant, Saccharomyces cerevisiae, antioxidant activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|